Coding
Part:BBa_K4706006:Design
Designed by: Niklas Küppers Group: iGEM23_Duesseldorf (2023-10-01)
E2A self cleaving peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For this part we started from the protein sequence and codon optimized it with online ordering tools in regard of synthesizing complexity. After that step we exchanged every rare codon (<16% usage) and replaced it with a more often used one with the help of Snapgenes algorithm. That way we received a good balance between synthesizing complexity and codon usage.
Source
BBa_K3544407
References
Tatiana M Souza-Moreira, Clara Navarrete, Xin Chen, Cleslei F Zanelli, Sandro R Valentini, Maysa Furlan, Jens Nielsen, Anastasia Krivoruchko, Screening of 2A peptides for polycistronic gene expression in yeast, FEMS Yeast Research, Volume 18, Issue 5, August 2018, foy036, https://doi.org/10.1093/femsyr/foy036